Purified IgG in phosphate buffered saline, pH 7.3.
Antibody come from
SIV-infected SupT1 cells injected into Balb/c mice.
Antibody's reacts with these species
This antibody doesn't cross react with other species
Test
You can block the antibody by the specific target amino acid sequence of peptide.
Warnings
This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. This datasheet is as accurate as reasonably achievable, but Nordic-MUbio accepts no liability for any inaccuracies or omissions in this information.
Antibody's suited for
For use in flow cytometry and immunohistochemical analyses of the CXCR4 receptor in human cell lines and tissues. Fixation with methanol and/or acetone was shown to destroy the epitope of the 12G5 mAb. 12G5 can be used to block SDF-1-induced chemotaxis and increases of intracellular calcium. The optimal antibody concentration should be determined for each specific application.
Description
Peptides short amino acid chains or epitopes or blocking antagonists. The shortest peptides are dipeptides, consisting of 2 amino acids joined by a single peptide bond, followed by tripeptides, tetra peptides, ... till polypeptides that are long, continuous, and unbranched synthetic peptide chains. These biological oligomers and polymers can be Solid-phase peptide synthesis (SPPS), or in continue produced for custom peptide synthesis projects. The High-efficiency solid phase peptide synthesis (HE-SPPS) is give very low production costs.
Properties
If you buy Antibodies supplied by nordc they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.blocking peptide,Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
Long description
Acetylation of the tail of histone is known to cause chromatin to adopt a more ÔopenÕ 3D conformation, allowing trans factors grerater access to DNA. Histone acetyltransferases (HATs), complexes interact with sequence-specific activator proteins and target specific genes. In addition to histones, HATs acetylate non-histone proteins, implicating them in a wide variety of regulartory roles for these enzymes. By comparison, histone deacetylation promotes a more ÔclosedÕ chromatin conformation and in general leads to repression of gene activity. Mammalian histone deacetylases are divided into three classes on the basis of their similarity to various the yeast deacetylases. Class I (HDACs 1, 2, 3 and 8) related to the yeast Rpd3-like proteins, class II (HDACs 4, 5, 6, 7, 9 and 10) related to yeast Hda1-like proteins and class III related to the yeast protein Sir2. Inhibitors of HDAC have enormous potential as cancer therapeutic agents.
Relevant references
1. Endres, M.J., Clapham, P.R., Marsh, M., Ahuja, M., Davis Turner, J., McKnight, A., Thomas, J.F., Stoebenau-Haggarty, B., Choe, S., Vance, P.J., Wells, T.N.C., Power, C.A., Sutterwala, S.S., Doms, R.W., Landau, N.R., and Hoxie, J.A. (1996) CD4independent infection of HIV-2 is mediated by Fusin/CXCR4. Cell 87:745-756. _x000B__x000B_2. Bleul, C.C., Wu, L., Hoxie, J.A., Springer, T.A., and Mackay, C.R. (1997) The HIV coreceptors CXCR4 and CCR5 are differentially expressed and regulated on human T lymphocytes. Proc. NatÕl. Acad. Sci. USA 94:1925-1930. _x000B__x000B_3. Taylor, J.R., Kimbrell, K.C., Scoggins, R., Delany, M., Wu, L. and Camerini, D. (2001) Expression and function of chemokine receptors on human thymocytes: Implications for infection by Human Immunodeficiency Virus Type I. J. Virol. Sept.:8752-8760. _x000B__x000B_4. Yoshino, N., Ami, Y., Terao, K., Tashiro, F. and Honda, M. (2000) Upgrading of flow cytometric analysis for absolute counts, cytokines and other antigenic molecules of cynomolgus monkeys (Macaca fascicularis) by using anti-human cross-reactive antibodies. Exp. Anim. 49(2):97-110.
