This antibody doesn't cross react with other species
Antibody come from
Synthetic peptide derived from the N-terminal of the human PINK1 protein.
Application
Western Blot, Functional epitope reported to block PCSK9-LDLR interaction. ELISA
Test
You can block the antibody by the specific target amino acid sequence of peptide.
Other description
Provided as solution in phosphate buffered saline with 0.08% sodium azide. Antigen Immunoaffiinity Purification
Antigen-antibody binding interaction
Rabbit anti Proprotein convertase subtilisin/kexin type 9 (PCSK9) antibody LDLR-binding epitope blocking Antibody
Antibody's suited for
Antibody can be used for immunohistochemical staining (1-5 µg/ml). Optimal concentration should be evaluated by serial dilutions.
Properties
If you buy Antibodies supplied by nordc they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
Warnings
This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. This datasheet is as accurate as reasonably achievable, but Nordic-MUbio accepts no liability for any inaccuracies or omissions in this information.
About
Rabbits are used for polyclonal antibody production by nordc. Rabbit antibodies are very stable and can be stored for several days at room temperature. nordc adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.
Long description
This anitbody is made to an epitope that is reported to block the PCSK9-LDLR (low density lipoprotein receptor) interaction. PCSK9 binds to the EGF-A domain of the LDLR and signals LDLR degradation. Reduced LDLR levels result in decreased LDL (low density lipid) metabolism leading to hypercholesterolemia. _x000B__x000B_Additioanlly, PCSK9 may be implicated in the differentiation of cortical neurons and may also play a role in cholesterol homeostasis. Defects in PCSK9 gene are the cause of familial hypercholesterolemia 3 (FH3). The protein is thought to play a central role in cholesterol homeostais
Relevant references
1. Matsuda, N., et al. ÔPINK1 stabilized by mitochondrial depolarization recruits Parkin to damaged mitochondria and activates latent Parkin for mitophagy.Õ J. Cell. Biol., 189, 211-221 (2010)_x000B__x000B_2. Vives-Bauza, C., et al. ÔPINK1-dependent recruitment of Parkin to mitochondria in mitophagy.Õ Proc. Natl. Acad. Sci. USA, 107, 378-383 (2010)_x000B__x000B_3. Valente, E.M., et al. ÔPINK1 mutations are associated with sporadic early-onset parkinsonism.Õ Ann. Neurol., 56, 336-341 (2004)_x000B__x000B_4. Song, S., et al. ÔCharacterization of PINK1 (PTEN-induced Putative Kinase 1) Mutations Associated with Parkinson Disease in Mammalian Cells and Drosophila.Õ J Biol Chem. 288(8): 5660-5672, (2013)
