This antibody doesn't cross react with other species
Antigen-antibody binding interaction
Sphingosine Kinase 1 antibody Long Form antibody Rat - Blocking Peptide
Other description
Provided as sterile filtered solution in phosphate buffered saline, pH 7.2.
Test
You can block the antibody by the specific target amino acid sequence of peptide.
Antibody come from
A 14 amino acid peptide corresponding to aa 5-19 at the N-terminus of human CXCR1
Properties
If you buy Antibodies supplied by nordc they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.blocking peptide
About
Rats are used to make rat monoclonal anti mouse antibodies. There are less rat- than mouse clones however. Rats genes from rodents of the genus Rattus norvegicus are often studied in vivo as a model of human genes in Sprague-Dawley or Wistar rats.
Warnings
This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. This datasheet is as accurate as reasonably achievable, but Nordic-MUbio accepts no liability for any inaccuracies or omissions in this information.
Antibody's suited for
This antibody is suitable for use in flow cytometry on isolated human neutrophils and on cells transfected with CXCR1. This antibody is also suitable for Western blotting, ELISA and immunohistochemistry. Incubation of human neutrophils with 1.0-4.0 µg/mL of clone 501 completely blocks IL-8-mediated chemotaxis.For flow cytometry applications, use 0.25-0.125 µg per 106 cells. The optimal antibody concentration should be determined for each specific application.
Relevant references
1. Lippert, U., et al. (1998) Expression and functional activity of the IL-8 receptor type CXCR1 and CXCR2 on human mast cells. J. Immunol. 161(5):2600-2608._x000B_2. Wuyts, A., et al. (1998) Differential usage of CXC chemokine receptors 1 and 2 by interleukin-8, granulocyte chemotactic protein-2 and epithelial-cell-derived neutrophil attractant-78. Eur. J. Biochem. 255(1):67-73._x000B_3. Wolf, M., et al. (1998) Granulocyte chemotactic protein 2 acts via both IL-8 receptors, CXCR1 and CXCR2. Eur. J. Immunol. 28(1):164-170._x000B_
Description
Peptides short amino acid chains or epitopes or blocking antagonists. The shortest peptides are dipeptides, consisting of 2 amino acids joined by a single peptide bond, followed by tripeptides, tetra peptides, ... till polypeptides that are long, continuous, and unbranched synthetic peptide chains. These biological oligomers and polymers can be Solid-phase peptide synthesis (SPPS), or in continue produced for custom peptide synthesis projects. The High-efficiency solid phase peptide synthesis (HE-SPPS) is give very low production costs.
Long description
Rat sphingosine kinase 1 catalyzes the phosphorylation of sphingosine to form sphingosine 1-phosphate (spp)(sphingosine + ATP = sphingosine 1-phosphate + ADP), a lipid mediator with both intra- and extracellular functions. also acts on d-erythro-sphingosine and to a lesser extent sphinganine, but not other lipids, such as d,l-threo-dihydrosphingosine, n,n-dimethylsphingosine, diacylglycerol, ceramide, or phosphatidylinositol. The long form of sphingosine kinase 1, an N-terminal extension, is responsible for FceRI-mediated calcium mobilization and may play a role in other ligand mediated signaling events.
